Producing monoclonal antibodies (4.3.2.1) | AQA GCSE Biology - Higher Tier - (8461) | KnowledgeSet

Producing monoclonal antibodies

Biology (Separate Science) · GCSE · AQA · AQA GCSE Biology - Higher Tier - (8461) · Specification point · 4.3.2.1 · Infection and response · Monoclonal antibodies (biology only)

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Learning Objectives

4.3.2.1 Producing monoclonal antibodies

Content	Key opportunities for skills development
$Students should be able to describe how monoclonal antibodies are produced. Monoclonal antibodies are produced from a single clone of cells. The antibodies are specific to one binding site on one protein antigen and so are able to target a specific chemical or specific cells in the body. They are produced by stimulating mouse lymphocytes to make a particular antibody. The lymphocytes are combined with a particular kind of tumour cell to make a cell called a hybridoma cell. The hybridoma cell can both divide and make the antibody. Single hybridoma cells are cloned to produce many identical cells that all produce the same antibody. A large amount of the antibody can be collected and purified.$

Content

Key opportunities for skills development

Students should be able to describe how monoclonal antibodies are produced. Monoclonal antibodies are produced from a single clone of cells. The antibodies are specific to one binding site on one protein antigen and so are able to target a specific chemical or specific cells in the body. They are produced by stimulating mouse lymphocytes to make a particular antibody. The lymphocytes are combined with a particular kind of tumour cell to make a cell called a hybridoma cell. The hybridoma cell can both divide and make the antibody. Single hybridoma cells are cloned to produce many identical cells that all produce the same antibody. A large amount of the antibody can be collected and purified.